Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1962; 08(03): 434-441DOI: 10.1055/s-0038-1655441 Originalarbeiten — Original Articles — Travaux Originaux Schattauer GmbH Procedures for the Quantitative Determination of Autoprothrombin C[*] Authors Author Affiliations Edmond R Cole 1 Department of Physiology and Pharmacology Wayne State University, College of Medicine, Detroit, Michigan, USA Ewa Marciniak ** 1 Department of Physiology and Pharmacology Wayne State University, College of Medicine, Detroit, Michigan, USA Walter H Seegers 1 Department of Physiology and Pharmacology Wayne State University, College of Medicine, Detroit, Michigan, USA Recommend Article Abstract PDF Download(opens in new window) Buy Article(opens in new window) Summary Two quantitative procedures for autoprothrombin C are described. In one of these purified prothrombin is used as a substrate, and the activity of autoprothrombin C can be measured even if thrombin is in the preparation. In this procedure a reaction mixture is used wherein the thrombin titer which develops in 20 minutes is proportional to the autoprothrombin C in the reaction mixture. A unit is defined as the amount which will generate 70 units of thrombin in the standardized reaction mixture. In the other method thrombin interferes with the result, because a standard bovine plasma sample is recalcified and the clotting time is noted. Autoprothrombin C shortens the clotting time, and the extent of this is a quantitative measure of autoprothrombin C activity. PDF (7788 kb) References References 1 Kowarzyk H, Marciniak E. The significance of prothrombin derivatives. Proceedings of the Eighth Congress of the European Society of Haematology, Wien, 1961. Karger, Basel; New York: 1962 2 Kowarzyk H, Marciniak E, Czerwinska B. Factor V and auto-prothrombin C. Arch. Immunol. Ter. dosw. 1961; 9: 719 3 Seegers W. H, Marciniak E, Cole E. R. Autocatalysis in prothrombin activation. Amer. J. Physiol. 1962; 203: 397 4 Marciniak E, Seegers W. H. Autoprothrombin C: A second enzyme from prothrombin. Canad. J. Biochem. 1962; 40: 597 5 Seegers W. H. The purification of prothrombin. Rec. Chem. Progr. 1952; 13: 143 6 Seegers W. H, Levine W. G, Shepard R. S. Further Studies on the purification of thrombin. Canad. J. Biochem. 1958; 36: 603 7 Ware A. G, Seegers W. H. Two-stage procedure for the quantitative determination of prothrombin concentration. Amer. J. clin. Path. 1949; 19: 471 8 Seegers W. H, Smith H. P. Factors which influence the activity of purified thrombin. Amer. J. Physiol. 1942; 137: 348 9 Seegers W. H, Marciniak E. Autoprothrombin C in irregular blood clotting. Thrombos. Diathes. haemorrh. 1962; 8: 1 10 Seegers W. H, Marciniak E. Inhibition of autoprothrombin C activity with plasma. Nature 1962; 193: 1188 11 Seegers W. H. Prothrombin. Harvard University Press; Cambridge, Massachusetts: 1962 12 Mertz E. T, Seegers W. H, Smith H. P. Prothrombin. thromboplastin and thrombin: Quantitative interrelationsships.. Proc. Soc. exp. Biol. (N. Y.) 1939; 42: 604 13 Hecht E, Landaburu R. H, Seegers W. H. Sphingosine as inhibitor of prothrombin activation with thromboplastin or with threone. Amer. J. Physiol. 1957; 189: 203
